VEGF (Vascular Endothelial Growth Factor) or VPF is the most important angiogenesis stimulator. It is a homodimeric, disulfide-linked glycoprotein which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.
Host Species: Rabbit
Buffer: 0.2% BSA and 0.09% sodium azide in PBS
Formulation: Purified rabbit IgG
Stability: The antibody is stable at 4°C for 24 months.
Cross-Reactivity: The antibody cross-reacts with human, mouse and rat VEGF. Other species not tested.
Specificity: The polyclonal antibody was raised against human VEGF165. The antibody works in immunostaining or immunohistochemistry of paraffin sections. It is recommended for rodent tissues. The sodium azide free form is good for neutralizing VEGF activity.
Immunostaining/Immunohistochemistry: Use 5-10µg/mlat RT 30 min. For paraffin embedded sections, boil tissue sections in 10 mM Tris buffer, pH 10 for 10-20 min followed by cooling at RT for 20 min to retrieve antigens.
Usage: This antibody is offered for research use only. Not for human use!
Boocock CA; et al. Journal of the National Cancer Institute, 1995 Apr 5, 87(7):506-16.
Brown LF; et al. Human Pathology, 1995 Jan, 26(1):86-91.