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anti-hVEGF (JH121)
Overview
Catalog #11-004
Unit Size100 ug
ApplicationsW, N, IHC(F/P) IF
Hostmouse
Price$350.00
Description:
VEGF (Vascular Endothelial Growth Factor) or VPF is the most important angiogenesis stimulator. It is a homodimeric, disulfide-linked glycoprotein which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.
 
Host Species: Mouse
Buffer: 0.1% BSA and 0.09% sodium azide in PBS
Formulation: Purified mouse IgG1
Stability: The antibody is stable at 4°C for 20 months.
 
Cross-Reactivity: The antibody cross-reacts with rabbit VEGF. Other species not tested.
 
Specificity: The monoclonal antibody is raised against human VEGF121 and recognizes all forms of VEGF. It is suitable for neutralizing the bioactivity of human VEGF (please order antibody without sodium azide). The antibody also works in immunostaining or immunohistochemistry and Western blotting.
 
Immunostaining/Immunohistochemistry: Use 1:50 dilution. For paraffin embedded sections, boil tissue sections in 1mM EDTA buffer, pH 8.0 for 10-20 min followed by cooling at RT for 20 min to retrieve antigens.
 
Western blotting: Use 1-2µg/ml
 
Usage: This antibody is offered for research use only. Not for human use!
 
References:
Boocock CA; et al. Journal of the National Cancer Institute, 1995 Apr 5, 87(7):506-16.
Brown LF; et al. Human Pathology, 1995 Jan, 26(1):86-91.
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