VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. It is an important regulator of the growth and/or differentiation of lymphatic endothelium. This antibody was produced from sera of rabbits immunized with highly pure recombinant rat dNdC-VEGF-C and was purified by affinity chromatography with immobilized Protein A.
Host Species: Rabbit
Formulation: Protein A purified rabbit IgG
Stability: The lyophilized IgG is stable at 4°C. for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freeze-thaw cycles.
Cross-Reactivity: The antibody cross-reacts with mouse and human VEGF-C. Other species not tested. .
Specificity: The antibody works in Western blot, IHC, ELISA and immunoprecipatation.
ELISA: To detect rat VEGF-C by direct ELISA a concentration of at least 2.5 µg/mlis required. This purified IgG in combination with compatible secondary reagents, allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C.
Immunoprecipitation: For IP use 1-10 µg IgG per 1 ml lysate or reaction volume.
Western blot, IHC: Use 1-10 µg/ml
Optimal dilutions should be determined by each laboratory for each application.
Usage: This antibody is offered for research use only. Not for human use!