Flk1(mouse)/KDR(human), or VEGFR2 is a high affinity receptor for VEGF and plays an important role in endothelial cell growth, differentiation and angiogenesis. It belongs to a family of receptor kinases. It contains an extracellular domain with seven immunoglobullin-like loops, a transmembrane domain and a cytoplasmic kinase domain.
Host Species: Rabbit
Buffer: 0.2% BSA and 0.09% sodium azide in PBS
Formulation: Purified rabbit IgG
Stability: The antibody is stable at 4°C for 24 months.
Cross-Reactivity: The antibody recognizes human, mouse and rat VEGFR2. Other species not tested.
Specificity: This antibody was raised against a synthetic peptide from the cytoplasmic domain of Flk1. It works in immunostaining or immunohistochemistry and Western blotting detecting a 180 kD band.
Immunostaining/Immunohistochemistry: Use 5-10µg/ml at RT for 30 min. For paraffin embedded sections, boil tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min to retrieve antigens.
Western blotting: Use 5-10µg/mlRT for 2 hrs
Optimal dilutions should be determined by each laboratory for each application.
Usage: This antibody is offered for research use only. Not for human use!
Quinn, TP, et al. (1993) Proc. Natl. Acad. Sci. USA, 90: 7533-7537