anti-human podoplanin NZ-1
Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. This rat monoclonal antibody (IgG2a) against human podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. Therefore, it will be a useful tool to study lymphatic vessel density in and around human tumors. This antibody also can be used in FACS sorting.
Host Species: Rat
Buffer: PBS contains 0.09% NaN3
Formulation: Purified rat IgG2a
Stability: The antibody is stable at 4°C for >six months or can be stored in working aliquots at -20°C for more than a year.
Cross-Reactivity: The antibody does not cross-react with mouse podoplanin. Other species not tested.
Specificity: The monoclonal antibody will detect human podoplanin on the surface of lymphatic endothelial cells and kidney podocytes by immunostaining or immunohistochemistry. It works well with paraffin sections. The antibody also works in Western blotting, immunoprecipitation and FACS sorting.
Immunostaining/Immunohistochemistry: Use 1:200 dilution
Western blotting: Use 1:200 dilution or more
Immunoprecipitation: For IP use 1:50 dilution
FACS: Use 1:200 dilution
Usage: This antibody is offered for research use only. Not for human use!