CD31 or PECAM-1 is a glycoprotein expressed on the cell surfaces of endothelial cells and platelets. It plays an important role in cell adhension. Anti-CD31 antibody is used to detect endothelial cells in tissue sections in applications such as measuring blood vessel density in tumors.
Host Species: Mouse
Buffer: Contains 0.1% NaN3
Formulation: Concentrated hybridoma supernatant
Stability: The antibody is stable at 4°C for >six month or can be stored in working aliquots at -20°C (add 50% glycerol) for more than a year.
Cross-Reactivity: The antibody recognizes human CD31. Other species not tested.
Specificity: The antibody was carefully tested for its ability to recognize human CD31. It works well with paraffin sections. For paraffin embedded sections, boil tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min to retrieve antigens. The antibody also works in FACS sorting.
Immunostaining/Immunohistochemistry: Use 1:50 dilution
FACS: Use 1:50 dilution
Optimal dilutions should be determined by each laboratory for each application.
Usage: This antibody is offered for research use only. Not for human use!