Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. This mouse monoclonal antibody (IgG) against human podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. Therefore, it will be a useful tool to study lymphatic vessel density in and around human tumors. This antibody also can be used in FACS sorting.
Host Species: mouse
Buffer: contains 0.09% NaN3
Formulation: Concentrated hybridoma supernatant
Stability: The antibody is stable at 4°C for >six month or can be stored in working aliquots at -20°C (add 50% glycerol) for more than a year.
Cross-Reactivity: The antibody does not cross-react with mouse podoplanin. Other species not tested.
Specificity: The monoclonal antibody will detect human podoplanin on the surface of lymphatic endothelial cells and kidney podocytes by immunostaining or immunohistochemistry. It works well with paraffin sections. The antibody also works in Western blotting, immunoprecipitation and FACS sorting.
Immunostaining/Immunohistochemistry: Use 1:50 to 1:200 dilution
Western blotting: Use 1:100 dilution or more
Immunoprecipitation: For IP use 1:50 dilution
FACS: Use 1:50 dilution
Usage: This antibody is offered for research use only. Not for human use!