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anti-Flt4(VEGFR3) (hu, mouse)
Overview
Catalog #11-015
Unit Size100 ug
ApplicationsW, IHC(F/P) IF
Hostrabbit
Price$300.00
Description:
Flt4 or VEGFR-3 is a high affinity receptor for VEGF-C and plays an important role in lymphangiogenesis. This antibody was produced from sera of rabbits immunized with recombinant human soluble VEGFR-3/FLT-4 (D1-D7) and purified by antigen-affinity chromatography with immobilized soluble VEGFR-3/FLT-4.
 
Host Species: Rabbit
Buffer: Lyophilized inPBS
Formulation: Antigen-affinity purified rabbit IgG
 
Stability: The lyophilized IgG is stable at 4°C. for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freeze-thaw cycles.
 
Cross-Reactivity: The antibody recognizes human VEGFR-3/FLT-4. Other species not tested.
 
Specificity: The antibody was carefully tested for its ability to recognise human VEGFR-3/FLT-4 in direct and indirect ELISA and Western blot assays. In Western blots, this antibody shows a moderate cross-reactivity with VEGFR-2/KDR and a weak cross-reactivity with VEGFR-1/Flt-1.
 
ELISA: To detect human VEGFR-3/FLT-4 by direct ELISA a concentration of 0.5 - 1.5 µg/ml can be used. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well hVEGFR-3/FLT-4
 
Western blotting: For Western blot analysis, the antibody can be used at 0.5 - 1 µg/ml with the appropriate secondary reagents to detect human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for hVEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions.
 
Optimal dilutions should be determined by each laboratory for each application.
 
Usage: This antibody is offered for research use only. Not for human use!
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